EloTrace®
EloTrace® – an automatic measurement system for electrooptical analysis of bacterial cultures in vivo
- On-line multiparametric monitoring of bacterial fermentations
- Ensure growth efficiency
- Reproducibility
- Ideal for documentation and scale-up
EloCheck
EloTrace® is an automatic measurement system for continuous electrooptical analysis of bacterial cultures in vivo. The electrooptical monitoring uses high-precision optical sensors which quantify induced polarized charges at cellular membranes. The level of this polarizability is closely linked to the actual physiological state of the cell, so that dynamic changes in cell activity and regulatory processes can be determined during cultivation processes in real time. No expensive chemical are needed. Intelligent software makes it easy to use.
Areas of application
- Production of recombinant proteins and other biosubstances
- Production of vaccines
- Production of starter cultures, probiotic and competent cells High density fermentation
- Quality assurance and process validation of fermentations
- Many other fermentation strategies
Advantages
- Automatic determination of
- Cell activity
- Stress levels
- Cell size
- Cell concentration
- Morphological changes
- Using high number of cell for statistically firm information
- Observation of division frequency and prediction of culture development
- Prediction of time points with higher cell vitality
- Selection of optimal time points of
- Inocculation
- Induction
- Harvest
- High precision data recording
- Automatic report generator
Electrical fields
- Frequencies: 10 kHz – 20 MHz
- Field strength: 20 – 100 V / cm
- Application time: 1.6 – 16.0 s
- Measurement chamber: approx. 0.4 – 8.0 ml
- Minimal cell concentration: 0.2 – 2 x 108 cells / ml
Vacuum
- Vacuum level 0.52 – 0.56 bar
Probe preparation
- Typical volume of suspension: 2 – 5 ml culture*
- Time for automatic probe preparation: 6 – 8 min*
*dependent on concentration of cells and remaining ions
Software
- Real-time data monitor
- Report function
- Data Export to ASCII, MS Excel or .pdf
Electrical characteristics
- External AC/DC adapter:
- Input: 220-230 V ~ (other Voltages available)
- 50-60 Hz, max. 1.5 A
- Output: 12 V = , max. 2.5 A
- EloTrace power usage: 12 V, max. 2.5 A
- Max. peak power 30 W
- PC Interface: RS232
Mechanical Parameters
- Dimensions (W ´ H ´ D): 492 ´ 370 ´ 410 mm
- Weight: 18.2 kg
Included in Delivery
- Electrooptical measurement unit
- Controller software installation CD
- Interface cable
- Power supply
- Vacuum station with water/waste management
- Filter, Tubes
- Instruction manual
Options
“Sample Dilutor” for pre-dilution and degassing of cell samples for electro-optical analysis in the “EloTrace”.
Especially recommended for bacterial fermentations with strong gassing and higher cell concentration (high cell density fermentation).
- Dilution factor, max. 5.2 – 6.0
- Application range, OD values > 120
- sterile sampling
- Automatic sterile cleaning of the module between sampling phases
Additional module works synchronously with EloTrace, removes from the fermenter, pre-dilutes and degasses the cell suspension.
Electrical characteristics
- External AC/DC adapter:
- Input: 220-230 V ~, 50-60 Hz, max. 1.5 A
- Output: 12 V = , max. 2.5 A
- PC interface: USB
Included in Delivery
- Sample Dilutor
- Fluid Connectors & Hoses
- Interface cable RS232
- Power supply unit 220V/12V DC
Mini PC or Notebook, Microsoft Windows Win 7 or Win 10 and system software “EloCheck” installed (Cat. no. Elo-001-03)
Technical Notes
not available
If you are interested in further information you can contact our service department using the contact form.